Journal: Scientific reports

Article Title: Galactin-8 DNA methylation mediates macrophage autophagy through the MAPK/mTOR pathway to alleviate atherosclerosis.

doi: 10.1038/s41598-024-85036-1

Figure Lengend Snippet: Fig. 4. (A) Detection of macrophage autophagosomes in aortic by projection electron microscopy. (B) Immunofluorescence localization of GAL8, LC3, MMP9, MCP-1 in mouse artery at 400 × magnification, Bar = 50 μm. Each group n = 6.

Article Snippet: These primary antibodies included GAL-8, TNF-α, MCP-1, MMP-9, Beclin1 (Proteintech, China, CatNo11-1AP), IL-1β (1:1000 dilution, 100 μL, Immunoway, China, Cat#YT2322), P62, LC3, P-MAPK (1:1000 dilution, 100 μL, Affinity, USA, Cat#AF4001), MAPK (1:100 dilution, 100 μL, Proteintech, China, Cat#14,064–1-AP), P-mTOR (1:1000 dilution, 100 μL, Proteintech, China, Cat#67,778–1-lg), mTOR (1:1000 dilution, 100 μL, Proteintech, China, Cat#: 67,778–1-lg), and DNMT1 (Biotechne, USA, Cat#NB100-56,519) at a standard dilution Scientific Reports | (2025) 15:603 3| https://doi.org/10.1038/s41598-024-85036-1 of 1:1000.

Techniques: Electron Microscopy, Immunofluorescence